TGT's OsteoGen bioreactors impart perfusion through cell seeded cylindrical scaffolds.  Applications include investigating cell function, modulating the growth and development of engineered tissues, or acting as a test bed for drug and regenerative medicine technologies.  The OsteoGen employs the 6Ocr-83 pulsatile hydrostatic pressure sitmulator  to deliver user defined sine pressure wave forms of physiological magnitude, with a maximum pressure of 200 mmHg. The 6Ocr-83 is integrated with a computer controlled mean flow pump and is capable of low or high flow configurations.  Researchers are currently utilizing these systems in a wide range of research areas including:

• Bone stem cell phenotype research
• Mineral deposition of marrowstromal cells

1O OsteoGen
The 1O system has been designed as a single sample bioreactor system that is able to support a wide range of  cell and tissue growth experiments via user defined stimulation protocols.  This chamber can accommodate a single sample with a maximum size of 10 mm diameter x 10 mm thick. Optional features such as; transducers, non-contact micrometers, pressure sensors, etc., and/or modules to customize the instrument to specific needs can be added to accomodate the research application.

12O OsteoGen
The 12O system has been designed as a single sample multi-chamber bioreactor system that is able to support a wide range of  cell and tissue growth experiments via user defined stimulation protocols.  This chamber can accommodate a single sample with a maximum size of 10 mm diameter x 10 mm thick. A custom manifold integrates up to 12 chambers on a common flow loop with a single stimulator and mean flow pump. However, if the application does not require pulsatile hydrostatic pressure the multi-chamber system can operate with individual flow loops. Optional features such as; transducers, non-contact micrometers, pressure sensors, etc., and/or modules to customize the instrument to specific needs can be added to accomodate the research application.

GrowthWorks® Software & Control:
A fifth generation design, the GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.

The GrowthWorks Control Hardware features an embedded Intel CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.

GrowthApps Stiffness Measurement & Adaptive Control:
The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the sample from initial seeding to an implantation-ready state with minimal operator intervention.

Click here to download a OsteoGen Brochure (Adobe PDF 21kb)